TY - JOUR
T1 - Hydrocarbon exposure effect on energetic metabolism and immune response in Crassostrea virginica
AU - López-Landavery, E. A.
AU - Amador-Cano, G.
AU - Tripp-Valdez, M. A.
AU - Ramírez-Álvarez, N.
AU - Cicala, F.
AU - Gómez-Reyes, R. J.E.
AU - Díaz, F.
AU - Re-Araujo, A. D.
AU - Galindo-Sánchez, C. E.
N1 - Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2022/7
Y1 - 2022/7
N2 - Crassostrea virginica was exposed to different light crude oil levels to assess the effect on transcriptomic response and metabolic rate. The exposure time was 21 days, and levels of 100 and 200 μg/L were used, including a control. The most significant difference among treatments was the overexpression of several genes associated with energy production, reactive oxygen species (ROS) regulation, immune system response, and inflammatory response. Also, a hydrocarbon concentration-related pattern was identified in ROS regulation, with a gene expression ratio near 1.8:1 between 200 and 100 μg/L treatments. Statistical analysis showed no interaction effect for metabolic rate; however, significant differences were found for oil concentration and time factors, with a higher oxygen consumption at 200 μg/L. Our findings provide novel information about the metabolic response of C. virginica during hydrocarbons exposure. In addition, our results point out which biological processes should be investigated as targets for searching bioindicators.
AB - Crassostrea virginica was exposed to different light crude oil levels to assess the effect on transcriptomic response and metabolic rate. The exposure time was 21 days, and levels of 100 and 200 μg/L were used, including a control. The most significant difference among treatments was the overexpression of several genes associated with energy production, reactive oxygen species (ROS) regulation, immune system response, and inflammatory response. Also, a hydrocarbon concentration-related pattern was identified in ROS regulation, with a gene expression ratio near 1.8:1 between 200 and 100 μg/L treatments. Statistical analysis showed no interaction effect for metabolic rate; however, significant differences were found for oil concentration and time factors, with a higher oxygen consumption at 200 μg/L. Our findings provide novel information about the metabolic response of C. virginica during hydrocarbons exposure. In addition, our results point out which biological processes should be investigated as targets for searching bioindicators.
KW - Gene expression
KW - Metabolic rate
KW - Oyster
KW - PAHs
KW - RNA-Seq
UR - http://www.scopus.com/inward/record.url?scp=85130902897&partnerID=8YFLogxK
U2 - 10.1016/j.marpolbul.2022.113738
DO - 10.1016/j.marpolbul.2022.113738
M3 - Artículo
C2 - 35635877
AN - SCOPUS:85130902897
SN - 0025-326X
VL - 180
JO - Marine Pollution Bulletin
JF - Marine Pollution Bulletin
M1 - 113738
ER -