TY - JOUR
T1 - Natural cortisol production is not linked to the sexual fate of European sea bass
AU - Goikoetxea, Alexander
AU - Servili, Arianna
AU - Houdelet, Camille
AU - Mouchel, Olivier
AU - Hermet, Sophie
AU - Clota, Fréderic
AU - Aerts, Johan
AU - Fernandino, Juan Ignacio
AU - Allal, François
AU - Vandeputte, Marc
AU - Blondeau-Bidet, Eva
AU - Geffroy, Benjamin
N1 - Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Nature B.V.
PY - 2022/8
Y1 - 2022/8
N2 - In this study, we aimed to investigate the relationship between cortisol and the determination of sexual fate in the commercially important European sea bass (Dicentrarchus labrax). To test our hypothesis, we designed two temperature-based experiments (19 ℃, 21 ℃ and 23 ℃, experiment 1; 16 ℃ and 21 ℃, experiment 2) to assess the effects of these thermal treatments on European sea bass sex determination and differentiation. In the fish from the first experiment, we evaluated whether blood cortisol levels and expression of stress key regulatory genes were different between differentiating (149 to 183 dph) males and females. In the second experiment, we assessed whether cortisol accumulated in scales over time during the labile period for sex determination as well as the neuroanatomical localisation of brain cells expressing brain aromatase (cyp19a1b) and corticotropin-releasing factor (crf) differed between males and females undergoing molecular sex differentiation (117 to 124 dph). None of the gathered results allowed to detect differences between males and females regarding cortisol production and regulatory mechanisms. Altogether, our data provide strong physiological, molecular and histochemical evidence, indicating that in vivo cortisol regulation has no major effects on the sex of European sea bass.
AB - In this study, we aimed to investigate the relationship between cortisol and the determination of sexual fate in the commercially important European sea bass (Dicentrarchus labrax). To test our hypothesis, we designed two temperature-based experiments (19 ℃, 21 ℃ and 23 ℃, experiment 1; 16 ℃ and 21 ℃, experiment 2) to assess the effects of these thermal treatments on European sea bass sex determination and differentiation. In the fish from the first experiment, we evaluated whether blood cortisol levels and expression of stress key regulatory genes were different between differentiating (149 to 183 dph) males and females. In the second experiment, we assessed whether cortisol accumulated in scales over time during the labile period for sex determination as well as the neuroanatomical localisation of brain cells expressing brain aromatase (cyp19a1b) and corticotropin-releasing factor (crf) differed between males and females undergoing molecular sex differentiation (117 to 124 dph). None of the gathered results allowed to detect differences between males and females regarding cortisol production and regulatory mechanisms. Altogether, our data provide strong physiological, molecular and histochemical evidence, indicating that in vivo cortisol regulation has no major effects on the sex of European sea bass.
KW - Cortisol
KW - European sea bass
KW - Sex determination
KW - Sex differentiation
KW - Temperature
UR - https://www.scopus.com/pages/publications/85135312311
U2 - 10.1007/s10695-022-01104-1
DO - 10.1007/s10695-022-01104-1
M3 - Artículo
C2 - 35917042
AN - SCOPUS:85135312311
SN - 0920-1742
VL - 48
SP - 1117
EP - 1135
JO - Fish Physiology and Biochemistry
JF - Fish Physiology and Biochemistry
IS - 4
ER -